Post-transcriptional regulation in higher eukaryotes: The role of the reporter gene in controlling expression

@article{Gallie1991PosttranscriptionalRI,
  title={Post-transcriptional regulation in higher eukaryotes: The role of the reporter gene in controlling expression},
  author={Daniel R Gallie and John N. Feder and Robert T. Schimke and Virginia Walbot},
  journal={Molecular and General Genetics MGG},
  year={1991},
  volume={228},
  pages={258-264}
}
We have investigated whether reporter genes influence cytoplasmic regulation of gene expression in tobacco and Chinese hamster ovary (CHO) cells. Two genes, uidA encoding β-glucuronidase (GUS) from Eseherichia coli and Luc, encoding firefly luciferase (LUC), were used to analyze the ability of a cap, polyadenylated tail, and the 5′- and 3′-untranslated regions (UTR) from tobacco mosaic virus (TMV) to regulate expression. The regulation associated with the 5′ cap structure and the TMV 5′-UTR… CONTINUE READING

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