Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender.

@article{MataCampuzano2015PostthawingQA,
  title={Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender.},
  author={Mar{\'i}a Mata-Campuzano and Manuel {\'A}lvarez-Rodr{\'i}guez and Mercedes Prudencio {\'A}lvarez and Julio Tamayo-Canul and Luis Anel and P L P{\'e}rez de Paz and F Mart{\'i}nez-Pastor},
  journal={Theriogenology},
  year={2015},
  volume={83 4},
  pages={
          520-8
        }
}
The performance of cryopreserved semen in ovine artificial insemination still needs improvement. Some antioxidants have been tested, with variable success. We cryopreserved semen from Churra rams using TES-Tris-fructose with 4% glycerol and 10% egg yolk (EY) or 3.5% soybean lecithin (SL), with 1 mM of reduced glutathione (GSH), Trolox, crocin, or cysteamine. Samples were analyzed after thawing and incubation (6 hours, 38 °C) for motility (computer-assisted sperm analysis [CASA]), viability… CONTINUE READING
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