Post-segregational killing by restriction modification gene complexes: observations of individual cell deaths.

@article{Handa1999PostsegregationalKB,
  title={Post-segregational killing by restriction modification gene complexes: observations of individual cell deaths.},
  author={Naofumi Handa and Issei Kobayashi},
  journal={Biochimie},
  year={1999},
  volume={81 8-9},
  pages={931-8}
}
Through a mechanism known as post-segregational killing, several plasmids mediate their stable maintenance by carrying genes that kill plasmid-free segregant cells. We demonstrated earlier that loss of plasmids carrying type II restriction modification (RM) gene complexes inhibits the propagation of a cell population and causes chromosome breakage. We now show the morphology of individual cells changes following loss of thermosensitive plasmids carrying EcoRI RM or PaeR7I RM after a shift to a… CONTINUE READING