Post-proline cleaving enzyme (prolyl endopeptidase) from bovine brain.

  title={Post-proline cleaving enzyme (prolyl endopeptidase) from bovine brain.},
  author={Tadashi Yoshimoto and Tomoaki Nishimura and Toshiyuki Kita and Daisuke Tsuru},
  journal={Journal of biochemistry},
  volume={94 4},
A post-proline cleaving enzyme [prolyl endopeptidase, EC] was purified about 3,700-fold from an extract of bovine brain by a series of column chromatographies on DEAE-Sephadex, hydroxyapatite and PCMB-T-Sepharose, and gel filtration on Sephadex G-200 using N-carbobenzoxy-Gly-Pro-beta-naphthylamide (Z-Gly-Pro-2-NNap), thyrotropin releasing hormone (TRH) and oxytocin as substrates. The purified enzyme appeared homogeneous as judged by disc gel and SDS gel electrophoreses. The enzyme was… CONTINUE READING
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