Post-integration stabilization of a transposon vector by terminal sequence deletion in Drosophila melanogaster

@article{Handler2004PostintegrationSO,
  title={Post-integration stabilization of a transposon vector by terminal sequence deletion in Drosophila melanogaster},
  author={Alfred M. Handler and Grazyna J. Zimowska and Carsten Horn},
  journal={Nature Biotechnology},
  year={2004},
  volume={22},
  pages={1150-1154}
}
Germline transformation systems for nearly 20 insect species have been derived from transposable elements, allowing the development of transgenic insects for basic and applied studies. These systems use a defective nonautonomous vector that results in stable vector integrations after the disappearance of transiently provided transposase helper plasmid, which is essential to maintain true breeding lines and consistent transgene expression that would otherwise be lost after vector remobilization… CONTINUE READING

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Transposable elements for insect transformation

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1 Excerpt

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