Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender.

@article{Machado2012PosthatchingDO,
  title={Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender.},
  author={Grazieli Marinheiro Machado and E S Caixeta and Carolina Madeira Lucci and Rodolfo Rumpf and Maur{\'i}cio Machaim Franco and Margot Alves Nunes Dode},
  journal={Zygote},
  year={2012},
  volume={20 2},
  pages={123-34}
}
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression of male and female IVP embryos that were cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5… CONTINUE READING
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Cellular and molecular deviations in bovine in vitro-produced are related to the large offspring syndrome

  • G. Lazzari, C. Wrenzycki, +4 authors C. Galli
  • Biol. Reprod
  • 2002
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