Post-conversion targeted capture of modified cytosines in mammalian and plant genomes

  title={Post-conversion targeted capture of modified cytosines in mammalian and plant genomes},
  author={Qing Li and Masako Suzuki and Jennifer A Wendt and Nicole E. Patterson and Steven R Eichten and Peter J. Hermanson and Dawn J Green and Jeffrey A. Jeddeloh and Todd A. Richmond and Heidi Rosenbaum and Daniel L. Burgess and Nathan M Springer and John M. Greally},
  booktitle={Nucleic acids research},
We present a capture-based approach for bisulfite-converted DNA that allows interrogation of pre-defined genomic locations, allowing quantitative and qualitative assessments of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at CG dinucleotides and in non-CG contexts (CHG, CHH) in mammalian and plant genomes. We show the technique works robustly and reproducibly using as little as 500 ng of starting DNA, with results correlating well with whole genome bisulfite sequencing data, and… CONTINUE READING


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