Post-Translational Regulation and Trafficking of the Granulin-Containing Protease RD21 of Arabidopsis thaliana

  title={Post-Translational Regulation and Trafficking of the Granulin-Containing Protease RD21 of Arabidopsis thaliana},
  author={Christian Gu and Mohammed Shabab and Richard Strasser and Pieter J Wolters and Takayuki Shindo and Melanie Niemer and Farnusch Kaschani and Lukas Mach and Renier A. L. van der Hoorn},
  journal={PLoS ONE},
RD21-like proteases are ubiquitous, plant-specific papain-like proteases typified by carrying a C-terminal granulin domain. RD21-like proteases are involved in immunity and associated with senescence and various types of biotic and abiotic stresses. Here, we interrogated Arabidopsis RD21 regulation and trafficking by site-directed mutagenesis, agroinfiltration, western blotting, protease activity profiling and protein degradation. Using an introduced N-glycan sensor, deglycosylation experiments… 

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