Post-Translational Regulation and Trafficking of the Granulin-Containing Protease RD21 of Arabidopsis thaliana

@article{Gu2012PostTranslationalRA,
  title={Post-Translational Regulation and Trafficking of the Granulin-Containing Protease RD21 of Arabidopsis thaliana},
  author={C. Gu and M. Shabab and R. Strasser and P. J. Wolters and T. Shindo and M. Niemer and Farnusch Kaschani and L. Mach and R. V. D. van der Hoorn},
  journal={PLoS ONE},
  year={2012},
  volume={7}
}
RD21-like proteases are ubiquitous, plant-specific papain-like proteases typified by carrying a C-terminal granulin domain. RD21-like proteases are involved in immunity and associated with senescence and various types of biotic and abiotic stresses. Here, we interrogated Arabidopsis RD21 regulation and trafficking by site-directed mutagenesis, agroinfiltration, western blotting, protease activity profiling and protein degradation. Using an introduced N-glycan sensor, deglycosylation experiments… Expand
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