Post-Transcriptional Gene Silencing of the p23 Silencing Suppressor of Citrus tristeza virus Confers Resistance to the Virus in Transgenic Mexican Lime

@article{Fagoaga2005PostTranscriptionalGS,
  title={Post-Transcriptional Gene Silencing of the p23 Silencing Suppressor of Citrus tristeza virus Confers Resistance to the Virus in Transgenic Mexican Lime},
  author={Carmen Fagoaga and Carmelo L{\'o}pez and Alfonso Hermoso de Mendoza and Pedro Ochoa Moreno and Luis Navarro and Ricardo Flores and Leandro Pe{\~n}a},
  journal={Plant Molecular Biology},
  year={2005},
  volume={60},
  pages={153-165}
}
Previously, we have shown that most Mexican limes (Citrus aurantifolia (Christ.) Swing.) expressing the p23 gene of Citrus tristeza virus (CTV) exhibit aberrations resembling viral leaf symptoms. Here we report that five independent transgenic lines having normal phenotype displayed characteristics typical of post-transcriptional gene silencing (PTGS): multiple copies of the transgene, low levels of the corresponding mRNA, methylation of the silenced transgene, and accumulation of p23-specific… CONTINUE READING

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