Post-PCR sterilization: development and application to an HIV-1 diagnostic assay

  title={Post-PCR sterilization: development and application to an HIV-1 diagnostic assay},
  author={S. T. Isaacs and J. W. Tessman and K. C. Metchette and J. E. Hearst and G. D. Cimino},
  journal={Nucleic acids research},
  volume={19 1},
We have developed an effective post-PCR sterilization process and have applied the procedure to a diagnostic assay for HIV-1. The method, which is based on isopsoralen photochemistry, satisfies both the inactivation and hybridization requirements of a practical sterilization process. The key feature of the technique is the use of isopsoralen compounds which form covalent photochemical adducts with DNA. These covalent adducts prevent subsequent extension of previously amplified sequences… CONTINUE READING


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Patent Application, in preparation

  • S. T. Isaacs, G. C. Cimino, K. C. Metchette, J. W. Tessman, U.S
  • J. Med Chem
  • 1989

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