Possible uncoupling of the mechanochemical process in the actomyosin system by covalent crosslinking of F-actin.

Abstract

It has been speculated that F-actin depolymerizes during muscle contraction. In order to test this speculation, G-actin in F-actins were immobilized by crosslinking. While this did not affect activation of myosin ATPase, superprecipitation could be abolished. Sedimentation, light scattering, electron microscope, electrophoresis and homodyne spectra… (More)

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