Possible role for Toll-like receptors in interaction of Fasciola hepatica excretory/secretory products with bovine macrophages.

Abstract

Alternative activation of macrophages (Mphi) during helminth infection is a characteristic feature of the host immune response. Alternatively activated macrophages (AAMphi) are distinguished from others by high arginase 1 (Arg-1) activity, low nitric oxide (NO), and high interleukin 10 (IL-10) production. In murine models, these cells have been shown to possess anti-inflammatory properties. They have also been implicated in exacerbating a subsequent infection with a secondary pathogen. In this study we used cattle experimentally infected with Fasciola hepatica to monitor the kinetics of IL-4 and IL-10 over the course of infection. Using naïve Mphi in vitro, we examined the effects of exposure to F. hepatica excretory/secretory products (FhepES) alone or in combination with IL-4. Our results suggest that FhepES may work in combination with IL-4 to produce AAMphi. The effects of FhepES on the subsequent responses to lipopolysaccharide (LPS) and purified protein derivative from Mycobacterium bovis (PPD-B), which are bovine Toll-like receptor 4 (TLR4) and TLR2 antagonists, respectively, were also examined. We found that Mphi stimulated with FhepES together with LPS or PPD-B have reduced NO or gamma interferon production, respectively. The ability of FhepES to produce AAMphi was found to be heat labile and partially dependent on glycan residues. A possible role for TLR recognition is discussed.

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@article{Flynn2008PossibleRF, title={Possible role for Toll-like receptors in interaction of Fasciola hepatica excretory/secretory products with bovine macrophages.}, author={Robin J Flynn and Grace M Mulcahy}, journal={Infection and immunity}, year={2008}, volume={76 2}, pages={678-84} }