Possible mechanism for in vitro complement activation in blood and plasma samples: futhan/EDTA controls in vitro complement activation.

@article{Pfeifer1999PossibleMF,
  title={Possible mechanism for in vitro complement activation in blood and plasma samples: futhan/EDTA controls in vitro complement activation.},
  author={P H Pfeifer and Masaaki Kawahara and Tony E. Hugli},
  journal={Clinical chemistry},
  year={1999},
  volume={45 8 Pt 1},
  pages={1190-9}
}
BACKGROUND Ongoing in vitro complement (C) activation in citrate or EDTA plasma has prevented an accurate analysis of C-activation products generated in vivo. The aim of this study was to characterize handling and storage conditions required to prevent in vitro C activation in blood and plasma samples collected with Futhan/EDTA. METHODS Biotrak(TM) RIAs were used to quantitatively measure C3a and C4a in blood and/or plasma samples from healthy individuals (controls) and from liver transplant… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 26 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 43 references

Distinct proteolytic activities of MASP-1 and MASP-2 [Abstract

  • M Matsushita, M Takahashi, S Thiel, JC Jensenius, T. Fujita
  • Mol Immunol 1998;35:349
  • 1999
1 Excerpt

EDTA blood/plasma samples may be caused by coagulation proteases

  • PH Pfeifer, TE Hugli, EW Davie, K. Fujikawa
  • Marshak DR, ed. Techniques in protein chemistry,
  • 1997

Measurement of complement C3a and C4a levels in liver transplant patients [Abstract

  • PH Pfeifer, JJ Brems, M Brunson, D Collins, TE. Hugli
  • The American Society of Transplant Surgeons 23rd…
  • 1997

Similar Papers

Loading similar papers…