Possible involvement of protein phosphorylation in the regulation of the sulphonylurea receptor of a pancreatic beta-cell line, HIT T15.

@article{Niki1991PossibleIO,
  title={Possible involvement of protein phosphorylation in the regulation of the sulphonylurea receptor of a pancreatic beta-cell line, HIT T15.},
  author={Ichiro Niki and Stephen J. H. Ashcroft},
  journal={Biochimica et biophysica acta},
  year={1991},
  volume={1133 1},
  pages={
          95-101
        }
}
The possible role of protein phosphorylation in modulation of [3H]glibenclamide binding to the sulphonylurea receptor, a putative ATP-sensitive K-channel, was investigated in the cloned pancreatic beta-cell line, HIT T15. Diazoxide, an opener of ATP-sensitive K-channels, increased HIT cell 86Rb-efflux, inhibited insulin secretion and decreased non-competitively [3H]glibenclamide binding to intact HIT cells. ATP-depletion reduced the [3H]glibenclamide binding activity of intact cells but did not… CONTINUE READING
BETA