Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system.

@article{Urao2000PossibleHT,
  title={Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system.},
  author={Takeshi Urao and Seiji Miyata and Kazuko Yamaguchi-Shinozaki and Kazuo Shinozaki},
  journal={FEBS letters},
  year={2000},
  volume={478 3},
  pages={227-32}
}
We have so far cloned a cDNA encoding a hybrid-type histidine kinase (ATHK1), three cDNAs encoding phosphorelay intermediates (ATHP1-3), and four cDNAs encoding response regulators (ATRR1-4) from Arabidopsis thaliana. To determine which molecules constitute a His to Asp phosphorelay pathway, we examined protein-protein interactions between them using a pairwise yeast two-hybrid analysis, as an initial step. We detected a specific interaction between ATHK1 and ATHP1. We further examined protein… CONTINUE READING
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