[Possibility of purifying cholera toxin (choleragen) and its antibodies by an immunosorption method].

Abstract

A study was made to establish the possibility of using an immune adsorbent based on polyacrylamide gel with the concentration of T = 10% and C = 25% for the purification of cholerogen and antibodies to it. For the isolation of cholera toxin the gamma globulin fraction of antitoxic serum was incorporated into the adsorbent; for obtaining antibodies an adsorbent with partially purified cholerogen was used. Desorption was made with 3 M sodium rhodanide solution. The yield of cholerogen was 41%, and the yield of antibodies was 4.5%. The non-specific sorption of the immune adsorbents was insignificant.

Cite this paper

@article{Zakrevskii1979PossibilityOP, title={[Possibility of purifying cholera toxin (choleragen) and its antibodies by an immunosorption method].}, author={V. I. Zakrevskii and V. I. Efremenko and Victor V. Lobanov and Ludmila Dolmatova}, journal={Zhurnal mikrobiologii, epidemiologii, i immunobiologii}, year={1979}, volume={5}, pages={30-4} }