Positive skin tests with autologous tumor cells of increased membrane viscosity


Skin tests were carried out with irradiated autologous tumor cells in patients with solid tumors (large-bowel carcinoma, gastric carcinoma, malignant melanoma, and breast cancer). With untreated cells, the skin reaction was negative or very weakly positive. A strong skin reaction, however, was elicited with cells of elevated membrane lipid microviscosity. This was induced by incorporation of cholesterol or, more effectively, by cholesterly hemisuccinate (CHS). A fair correlation was observed between the level of skin reaction with CHS-treated cells and the clinical stage of the tumor. It is suggested that rigidification of the cell membrane lipid layer exposes latent tumor-associated antigens, which enhances the cell-specific tumor immunogenicity. CHS-treated cells could therefore be of clinical value in active immunotherapy of human cancer.

DOI: 10.1007/BF00199259

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@article{Skornick2004PositiveST, title={Positive skin tests with autologous tumor cells of increased membrane viscosity}, author={Yehuda Skornick and E. Danciger and Raphael Rozin and Meir Shinitzky}, journal={Cancer Immunology, Immunotherapy}, year={2004}, volume={11}, pages={93-96} }