Positive-selection vectors using the F plasmid ccdB killer gene.

  title={Positive-selection vectors using the F plasmid ccdB killer gene.},
  author={P M Bernard and Philippe Gabant and El Mustapha Bahassi and Martine Couturier},
  volume={148 1},
Plasmids pKIL18/19 are positive-selection cloning vectors containing an active cytotoxic ccdB gene under the control of the lacP promoter. They are derivatives of high-copy-number pUC18/19 plasmids in which the ccdB killer gene has been fused in phase downstream from the lacP MCS18 and MCS19 multiple cloning sites. When an Escherichia coli wild-type gyrA+ strain is transformed by such vectors, the ccdB gene product blocks bacterial growth. However, if ccdB is inactivated by insertion of a… CONTINUE READING