Positive selection of mutations leading to loss or reduction of transcriptional activity of PrfA, the central regulator of Listeria monocytogenes virulence.

@article{Herler2001PositiveSO,
  title={Positive selection of mutations leading to loss or reduction of transcriptional activity of PrfA, the central regulator of Listeria monocytogenes virulence.},
  author={Michael Herler and Andreas Bubert and Monika Goetz and Yolanda Vega and Jose Vazquez-Boland and W. Goebel},
  journal={Journal of bacteriology},
  year={2001},
  volume={183 19},
  pages={
          5562-70
        }
}
Transcription factor PrfA controls the expression of virulence genes essential for Listeria monocytogenes pathogenesis. To gain insight into the structure-function relationship of PrfA, we devised a positive-selection system to isolate mutations reducing or abolishing transcriptional activity. The system is based on the observation that the listerial iap gene, encoding the p60 protein, is lethal if overexpressed in Bacillus subtilis. A plasmid in which the iap gene is placed under the control… CONTINUE READING

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