Corpus ID: 28216267

Positive effectors of the binding of an active site-directed amino steroid to rabbit cytochrome P-450 3c.

@article{Johnson1988PositiveEO,
  title={Positive effectors of the binding of an active site-directed amino steroid to rabbit cytochrome P-450 3c.},
  author={E. F. Johnson and G. Schwab and L. Vickery},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 33},
  pages={
          17672-7
        }
}

E. F. Johnson, G. Schwab, L. Vickery

Published 1988

Chemistry, Medicine

The Journal of biological chemistry

The binding of the amino steroid, 22-amino-23,24-bisnor-5-cholen-3 beta-ol (22-ABC), to rabbit liver cytochrome P-450 3c was studied using purified P-450 3c and liver microsomes prepared from rifampicin-treated B/J rabbits. 22-ABC binds to purified cytochrome P-450 3c producing a type II spectral change reflecting the coordination of the amine with the heme iron of the protein. In the absence of allosteric effectors, the binding is characterized by a Ks of 5 microM. In the presence of alpha… CONTINUE READING

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