Positional effect of chemical modifications on short interference RNA activity in mammalian cells.

@article{Prakash2005PositionalEO,
  title={Positional effect of chemical modifications on short interference RNA activity in mammalian cells.},
  author={Thazha P. Prakash and Charles R. Allerson and Prasad Dande and Timothy A. Vickers and Namir B Sioufi and Russell Jarres and Brenda F. Baker and Eric E Swayze and Richard H. Griffey and B. Shyamasundara Bhat},
  journal={Journal of medicinal chemistry},
  year={2005},
  volume={48 13},
  pages={4247-53}
}
A systematic study on the effect of 2'-sugar modifications (2'-F (2'-F-2'-deoxy-nucleoside residues), 2'-O-Me (2'-O-methyl-nucleoside residues), and 2'-O-MOE [2'-O-(2-methoxyethyl)]-nucleoside residues) in the antisense and sense strands of short interference RNA (siRNA) was performed in HeLa cells. The study of the antisense strand of siRNAs demonstrated that activity depends on the position of the modifications in the sequence. The siRNAs with modified ribonucleotides at the 5'-end of the… CONTINUE READING

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