Position-specific trapping of topoisomerase I-DNA cleavage complexes by intercalated benzo[a]- pyrene diol epoxide adducts at the 6-amino group of adenine.

@article{Pommier2000PositionspecificTO,
  title={Position-specific trapping of topoisomerase I-DNA cleavage complexes by intercalated benzo[a]- pyrene diol epoxide adducts at the 6-amino group of adenine.},
  author={Yves Pommier and Gary S. Laco and Glenda Kohlhagen and Jane M. Sayer and Heiko Kroth and Donald M. Jerina},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 20},
  pages={10739-44}
}
DNA topoisomerase I (top1) is the target of potent anticancer agents, including camptothecins and DNA intercalators, which reversibly stabilize (trap) top1 catalytic intermediates (cleavage complexes). The aim of the present study was to define the structural relationship between the site(s) of covalently bound intercalating agents, whose solution conformations in DNA are known, and the site(s) of top1 cleavage. Two diastereomeric pairs of oligonucleotide 22-mers, derived from a sequence used… CONTINUE READING
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Molecular Cloning: A Laboratory Manual (Cold

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