Position-specific chemical modification and quantitative proteomics disclose protein orientation adsorbed on silica nanoparticles.


We describe a method for determining the orientation of cytochrome c, RNase A, and lysozyme on silica nanoparticles (SNPs) using chemical modification combined with proteolysis-mass spectrometry. The proteins interacted with SNPs through preferential adsorption sites, which are dependent on SNP diameter; 4 nm SNPs induce greater structural stabilization… (More)
DOI: 10.1021/nl2044524


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