Position-effect protection and enhancer blocking by the chicken beta-globin insulator are separable activities.

@article{RecillasTarga2002PositioneffectPA,
  title={Position-effect protection and enhancer blocking by the chicken beta-globin insulator are separable activities.},
  author={F{\'e}lix Recillas-Targa and Michael J. Pikaart and Bonnie Burgess-Beusse and A Cheria Bell and Michael D Litt and Adam G West and Mikl{\'o}s Gaszner and Gary Felsenfeld},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 10},
  pages={6883-8}
}
The 1.2-kb DNA sequence element (5'HS4) at the 5' end of the chicken beta-globin locus has the two defining properties of an insulator: it prevents an "external" enhancer from acting on a promoter when placed between them ("enhancer blocking") and acts as a barrier to chromosomal position effect (CPE) when it surrounds a stably integrated reporter. We previously reported that a single CTCF-binding site in 5'HS4 is necessary and sufficient for enhancer blocking. We show here that a 250-bp "core… CONTINUE READING

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