Position-dependent inhibition of class-switch recombination by PGK-neor cassettes inserted into the immunoglobulin heavy chain constant region locus.

@article{Seidl1999PositiondependentIO,
  title={Position-dependent inhibition of class-switch recombination by PGK-neor cassettes inserted into the immunoglobulin heavy chain constant region locus.},
  author={K. J. Seidl and J. Manis and A. Bottaro and J. Zhang and L. Davidson and A. Kisselgof and H. Oettgen and F. Alt},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 6},
  pages={
          3000-5
        }
}
  • K. J. Seidl, J. Manis, +5 authors F. Alt
  • Published 1999
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • The Ig heavy chain (IgH) constant region (CH) genes are organized from 5' to 3' in the order Cmicro, Cdelta, Cgamma3, Cgamma1, Cgamma2b, Cgamma2a, Cepsilon, and Calpha. Expression of CH genes downstream of Cdelta involves class-switch recombination (CSR), a process that is targeted by germ-line transcription (GT) of the corresponding CH gene. Previously, we demonstrated that insertion of a PGK-neor cassette at two sites downstream of Calpha inhibits, in cultured B cells, GT of and CSR to a… CONTINUE READING
    87 Citations
    Elucidation of a downstream boundary of the 3' IgH regulatory region.
    • 25
    Germ line transcription in mice bearing neor gene downstream of Igamma3 exon in the Ig heavy chain locus.
    • 7
    • Highly Influenced
    • PDF
    Switch recombination and somatic hypermutation are controlled by the heavy chain 3′ enhancer region
    • 72
    • PDF

    References

    SHOWING 1-10 OF 52 REFERENCES
    Class Switching in B Cells Lacking 3′ Immunoglobulin Heavy Chain Enhancers
    • 141
    • PDF
    Replacement of germ-line epsilon promoter by gene targeting alters control of immunoglobulin heavy chain class switching.
    • 110
    • PDF