Position-dependent inhibition of class-switch recombination by PGK-neor cassettes inserted into the immunoglobulin heavy chain constant region locus.

@article{Seidl1999PositiondependentIO,
  title={Position-dependent inhibition of class-switch recombination by PGK-neor cassettes inserted into the immunoglobulin heavy chain constant region locus.},
  author={Katherine J. Seidl and John P Manis and Andrea Bottaro and Jun Yu Zhang and Laurie Wayne Davidson and Alison B. Kisselgof and Herbert Friedrich Oettgen and Frederick W Alt},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 6},
  pages={
          3000-5
        }
}
The Ig heavy chain (IgH) constant region (CH) genes are organized from 5' to 3' in the order Cmicro, Cdelta, Cgamma3, Cgamma1, Cgamma2b, Cgamma2a, Cepsilon, and Calpha. Expression of CH genes downstream of Cdelta involves class-switch recombination (CSR), a process that is targeted by germ-line transcription (GT) of the corresponding CH gene. Previously, we demonstrated that insertion of a PGK-neor cassette at two sites downstream of Calpha inhibits, in cultured B cells, GT of and CSR to a… CONTINUE READING

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