Position and orientation of the globular domain of linker histone H5 on the nucleosome

It is essential to identify the exact location of the linker histone within nucleosomes, the fundamental packing units of chromatin, in order to understand how condensed, transcriptionally inactive chromatin forms. Here, using a site-specific protein–DNA photo-crosslinking method, we map the binding site and the orientation of the globular domain of linker… CONTINUE READING