Portable microsatellite primers for Ficus (Moraceae).

  title={Portable microsatellite primers for Ficus (Moraceae).},
  author={Marjorie Garcia and Anthony Bain and Hsy-Yu Tzeng and Yan-qiong Peng and Lien-Siang Chou and Finn Kjellberg},
  journal={American journal of botany},
  volume={99 5},
PREMISE OF THE STUDY Highly portable microsatellite primers were developed for Ficus to facilitate investigation of genetic structure of complete regional floras using a single set of markers. METHODS AND RESULTS Pyrosequencing of five species of Ficus produced a library of 5723 potential primers. Potential primers found in at least two species and presenting identical annealing temperatures were tested on a set of five additional Ficus species. A set of 20 primer pairs producing well-defined… CONTINUE READING