Portable, Constriction-Expansion Blood Plasma Separation and Polymerization-Based Malaria Detection.

Abstract

A portable, microfluidic blood plasma separation device is presented featuring a constriction-expansion design, which produces 100.0% purity for undiluted blood at 9% yield. This level of purity represents an improvement of at least 1 order of magnitude with increased yield compared to that achieved previously using passive separation. The system features high flow rates, 5-30 μL/min plasma collection, with minimal clogging and biofouling. The simple, portable blood plasma separation design is hand-driven and can easily be incorporated with microfluidic or laboratory scale diagnostic assays. The separation system was applied to a paper-based diagnostic test for malaria that produced an amplified color change in the presence of Plasmodium falciparum histidine-rich protein 2 at a concentration well below clinical relevancy for undiluted whole blood.

DOI: 10.1021/acs.analchem.6b01355

Cite this paper

@article{Shatova2016PortableCB, title={Portable, Constriction-Expansion Blood Plasma Separation and Polymerization-Based Malaria Detection.}, author={Tatyana A Shatova and Shefali Lathwal and Marissa R Engle and Hadley D. Sikes and Klavs F Jensen}, journal={Analytical chemistry}, year={2016}, volume={88 15}, pages={7627-32} }