Porphyromonas gingivalis‐induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase

@article{Naito2006PorphyromonasGP,
  title={Porphyromonas gingivalis‐induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase},
  author={M. Naito and E. Sakai and Yixin Shi and H. Ideguchi and M. Shoji and N. Ohara and Kenji Yamamoto and K. Nakayama},
  journal={Molecular Microbiology},
  year={2006},
  volume={59}
}
Evidence from recent epidemiological studies suggests a link between periodontal infections and increased risk of atherosclerosis and related cardiovascular and cerebrovascular events in human subjects. One of the major pathogens of periodontitis, Porphyromonas gingivalis, has the ability to aggregate human platelets in platelet‐rich plasma (PRP). Mechanism of P. gingivalis‐induced platelet aggregation in PRP was investigated. Proteinase inhibitors toward Arg‐gingipain (Rgp) and Lys‐gingipain… Expand
Porphyromonas gingivalis-induced Platelet Aggregation in Human Plasma
Abstract The periodontal pathogen Porphyromonas gingivalis has the ability to aggregate human platelets. In this study, the mechanism of P. gingivalis -induced platelet aggregation in platelet-richExpand
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TLDR
This study shows that primary features of platelet activation, i.e. changes in intracellular free calcium and aggregation, are affected by P. gingivalis and that arg-gingipains are of great importance for the ability of the bacterium to activate platelets, and further clarify platelets as active players in innate immunity and in sensing bacterial infections. Expand
Platelet plug formation in whole blood is enhanced in the presence of Porphyromonas gingivalis.
TLDR
Assessment of platelet responses to the presence of P. gingivalis in whole blood found that the bacterium enhances thrombus forming potential of platelets in wholeBlood. Expand
Porphyromonas gingivalis cell-induced hemagglutination and platelet aggregation.
TLDR
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Molecular mechanisms of Porphyromonas gingivalis-host cell interaction on periodontal diseases
TLDR
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Selective proteolysis of apolipoprotein B-100 by Arg-gingipain mediates atherosclerosis progression accelerated by bacterial exposure.
TLDR
Results indicate that degradation of apoB-100 by Rgp plays a crucial role in the promotion of atherosclerosis by P. gingivalis infection. Expand
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References

SHOWING 1-10 OF 71 REFERENCES
Porphyromonas gingivalis platelet aggregation activity: outer membrane vesicles are potent activators of murine platelets.
TLDR
Data show that initial adherence of the bacterium to platelet may be facilitated by P. gingivalis fimbriae, and that vesicles (outer membrane evaginations that are shed into the environment by the bacteria) of P.GingivalIS are potent inducers of mouse platelet aggregation in vitro. Expand
Activation of protease-activated receptors by gingipains from Porphyromonas gingivalis leads to platelet aggregation: a new trait in microbial pathogenicity.
TLDR
Results indicate the existence of a novel pathway of host cell activation by bacterial proteinases through PAR cleavage, which not only represents a new trait in bacterial pathogenicity, but may also explain an emerging link between periodontitis and cardiovascular disease. Expand
A role for glycoprotein Ib in Streptococcus sanguis-induced platelet aggregation.
TLDR
It is suggested that the interaction between S sanguis and GPIb is important in the pathogenesis of infective endocarditis and may also play a contributory role in some cases of myocardial infarction. Expand
Pathogenesis of periodontitis: a major arginine-specific cysteine proteinase from Porphyromonas gingivalis induces vascular permeability enhancement through activation of the kallikrein/kinin pathway.
TLDR
Results indicate that RGP-1 is the major VPE factor of P. gingivalis, inducing this activity through PK activation and subsequent BK release, resulting in GCF production at sites of periodontitis caused by infection with this organism. Expand
Helicobacter pylori binds von Willebrand factor and interacts with GPIb to induce platelet aggregation.
TLDR
The interaction with platelets appeared to be mediated by vWF because platelet aggregation was blocked by an antibody to vWF, and a strain of H. pylori that induced Platelet aggregation bound vWF to a greater extent than a nonaggregating strain. Expand
Proteolysis of Human Monocyte CD14 by Cysteine Proteinases (Gingipains) from Porphyromonas gingivalis Leading to Lipopolysaccharide Hyporesponsiveness1
TLDR
It is suggested that gingipains preferentially cleave monocyte CD14, resulting in attenuation of the cellular recognition of bacteria, and as a consequence sustain chronic inflammation. Expand
Inactivation of Tumor Necrosis Factor-α by Proteinases (Gingipains) from the Periodontal Pathogen, Porphyromonas gingivalis
TLDR
Results indicate that the gingipain proteinases elaborated by P. gingivalis are capable of disrupting the cytokine network at the site of infection through the degradation of the proinflammatory cytokine TNF-α, suggesting the removal of one of several mediators important to the function of polymorphonuclear leukocytes. Expand
Roles for Arg- and Lys-Gingipains in the Disruption of Cytokine Responses and Loss of Viability of Human Endothelial Cells by Porphyromonas gingivalis Infection
TLDR
Findings indicate that P. gingivalis modulates the cytokine response in the cells and disrupts the adhesion activity and the viability through the cooperative action of Rgp and Kgp and thereby may contribute to pathogenesis of cardiovascular diseases as well as periodontal disease. Expand
Proteolytic inactivation of the leukocyte C5a receptor by proteinases derived from Porphyromonas gingivalis
TLDR
These studies suggest that P. gingivalis contains at least two enzymes capable of cleaving the C5aR, Lys-gingipain and a second nontryptic serine proteinase that is distinct from either Arg- or Lys-gesipain. Expand
Platelet activation by Protease I of Porphyromonas gingivalis W83.
TLDR
It is concluded that the activation of platelets by P. gingivalis Protease I involves proteolysis, but not scission of theThrombin cleavage site of the thrombin receptor. Expand
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