Porphyrin fluorescence dominates UV photoemission of folded cytochrome c.

@article{Lwenich2007PorphyrinFD,
  title={Porphyrin fluorescence dominates UV photoemission of folded cytochrome c.},
  author={Dennis L{\"o}wenich and Karl Kleinermanns},
  journal={Photochemistry and photobiology},
  year={2007},
  volume={83 6},
  pages={1308-12}
}
In this article we reinvestigate the bimodal fluorescence of cytochrome c (Cyt c) by using excitation-wavelength-dependent fluorescence spectroscopy. We show that its major contributions at pH 3-7 do not arise from tryptophan (Trp-59) fluorescence as hitherto assumed. Instead, different chromophores of Cyt c contribute at different pH values. At pH 3-7, the porphyrin system contributes about 80% and tryptophan about 20% to the total fluorescence upon excitation of Cyt c at 280 nm. At pH 2, the… CONTINUE READING

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