Porcine reproductive and respiratory syndrome virus (PRRSV): monoclonal antibodies detect common epitopes on two viral proteins of European and U.S. isolates.

@article{WieczorekKrohmer1996PorcineRA,
  title={Porcine reproductive and respiratory syndrome virus (PRRSV): monoclonal antibodies detect common epitopes on two viral proteins of European and U.S. isolates.},
  author={Martina Wieczorek-Krohmer and Frank Weiland and Karl Klaus Conzelmann and Debra Kohl and Marjolein Visser and Peter A van Woensel and H. Jurgen Thiel and Emilie Weiland},
  journal={Veterinary microbiology},
  year={1996},
  volume={51 3-4},
  pages={
          257-66
        }
}
Sixteen hybridoma cell lines secreting monoclonal antibodies (mAbs) directed against two dutch isolates of the causative virus of Porcine Reproductive and Respiratory Syndrome (PRRSV) were produced. The hybridoma cells resulted from fusions of SP2/0 myeloma cells with splenocytes of STU mice immunized with purified PRRSV after induction of immunotolerance against host cell constituents. Screening of supernatant fluids was performed by an indirect immunofluorescence assay on PRRSV-infected… CONTINUE READING
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