Porcine follicular fluid contains several low molecular weight inhibitors of follicle-stimulating hormone binding to receptor.

@article{Sluss1984PorcineFF,
  title={Porcine follicular fluid contains several low molecular weight inhibitors of follicle-stimulating hormone binding to receptor.},
  author={Patrick M. Sluss and Leo E Reichert},
  journal={Biology of reproduction},
  year={1984},
  volume={30 5},
  pages={1091-104}
}
Porcine follicular fluid (PFF) inhibited the binding of 125I-human follicle-stimulating hormone (hFSH) to receptor in vitro in a dose-dependent fashion. PFF (2.5 l) was fractionated on the basis of apparent molecular weight (Mr) by ultrafiltration using hollow fibers and membranes of precalibrated pore size. Desalted, low Mr (500-5000) subfractions containing FSH-binding inhibitor (FSH-BI) activity were further purified by Sephadex G10 gel filtration and anion-exchange high-performance liquid… CONTINUE READING