Porcine cardiac valvular subendothelial cells in culture: cell isolation and growth characteristics.

@article{Johnson1987PorcineCV,
  title={Porcine cardiac valvular subendothelial cells in culture: cell isolation and growth characteristics.},
  author={C. M. Johnson and Mark Nils Hanson and S C Helgeson},
  journal={Journal of molecular and cellular cardiology},
  year={1987},
  volume={19 12},
  pages={
          1185-93
        }
}
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100 Citations
Highly Influential Citations
4
Background Citations
23
Methods Citations
28

100 Citations

Smoothelin-positive cells in human and porcine semilunar valves

The examination of valve interstitial cells confirmed the presence of terminally differentiated, contractile smooth muscle cells in situ and suggested that smooth muscle α-actin and smoothelin interact, as has been previously postulated.

Robust Generation of Quiescent Porcine Valvular Interstitial Cell Cultures

The approach using a fibroblast media formulation with culture on collagen coatings generates quiescent VICs that more accurately mimic a healthy VIC population and thus has the potential to transform the study of the mechanisms of VIC activation and dysfunction involved in the early stages of calcific aortic valve disease.

Bovine mitral valve organ culture: role of interstitial cells in repair of valvular injury.

Fibronectin-based isolation of valve interstitial cell subpopulations: relevance to valve disease.

Myxomatous mitral valves (MVs) contain elevated proportions of unique cell populations such as myofibroblasts. Without a reliable technique to isolate such cell populations, however, it has been…

Interstitial cells from the atrial and ventricular sides of the bovine mitral valve respond differently to denuding endocardial injury

In organ culture, interstitial cells from the ventricular side of the mitral valve respond to a denuding endocardial injury by proliferating and migrating onto the adjacent surface whereas interstitial Cells from the atrial side do not.

Characterization of Cell Subpopulations Expressing Progenitor Cell Markers in Porcine Cardiac Valves

Cell profiling based on flow cytometry and functional studies with sorted primary cells provide not only new and quantitative information about the cellular composition of porcine cardiac valves, but also contribute to the understanding of how a subpopulation of valvular cells (ABCG2+ cells) may participate in tissue repair and disease progression.

Serum deprivation improves seeding and repopulation of acellular matrices with valvular interstitial cells.

A reduction of serum content was found to increase significantly the number of adherent cells, as well as induce transfer of VICs from a tissue-culture polystyrene surface to the aScaffold, leading to a uniformly repopulated valve leaflet construct after 4 weeks of static culture.

Leaflet Interstitial Cells

This chapter will focus on studies of atrioventricular and aortic valve LIC in pig, rabbit, hamster, rat, mouse, and human, and the structural and functional characteristics of these cells.

Characterization of Porcine Aortic Valvular Interstitial Cell ‘Calcified’ Nodules

It is demonstrated that ‘calcified’ nodules formed from PAVICs grown in OST+TGF-β1 medium do not mineralize after 21 days in culture, but rather they express a myofibroblast-like phenotype and produce a collagen-rich extracellular matrix.

Substrate properties influence calcification in valvular interstitial cell culture.

Both, the biochemical and mechanical environment of tissue culture has an effect on the spontaneous calcification of VICs, and may also have a profound effect on their molecular properties, as related to an understanding of the disease process in vivo.
...

References

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