Porcine cardiac valvular subendothelial cells in culture: cell isolation and growth characteristics.

@article{Johnson1987PorcineCV,
  title={Porcine cardiac valvular subendothelial cells in culture: cell isolation and growth characteristics.},
  author={C. M. Johnson and Mark Nils Hanson and S C Helgeson},
  journal={Journal of molecular and cellular cardiology},
  year={1987},
  volume={19 12},
  pages={
          1185-93
        }
}
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99 Citations
Highly Influential Citations
4
Background Citations
22
Methods Citations
28

99 Citations

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Robust Generation of Quiescent Porcine Valvular Interstitial Cell Cultures
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Myxomatous mitral valves (MVs) contain elevated proportions of unique cell populations such as myofibroblasts. Without a reliable technique to isolate such cell populations, however, it has been…
Modulation of Human Valve Interstitial Cell Phenotype and Function Using a Fibroblast Growth Factor 2 Formulation
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Treatment of human Vics with this fibroblast media formulation has the ability to maintain and to dedifferentiate the VICs back to a fibroblastic phenotype with phenotypic and functional characteristics ascribed to cells in the intact valve.
Interstitial cells from the atrial and ventricular sides of the bovine mitral valve respond differently to denuding endocardial injury
TLDR
In organ culture, interstitial cells from the ventricular side of the mitral valve respond to a denuding endocardial injury by proliferating and migrating onto the adjacent surface whereas interstitial Cells from the atrial side do not.
Characterization of Cell Subpopulations Expressing Progenitor Cell Markers in Porcine Cardiac Valves
TLDR
Cell profiling based on flow cytometry and functional studies with sorted primary cells provide not only new and quantitative information about the cellular composition of porcine cardiac valves, but also contribute to the understanding of how a subpopulation of valvular cells (ABCG2+ cells) may participate in tissue repair and disease progression.
Serum deprivation improves seeding and repopulation of acellular matrices with valvular interstitial cells.
TLDR
A reduction of serum content was found to increase significantly the number of adherent cells, as well as induce transfer of VICs from a tissue-culture polystyrene surface to the aScaffold, leading to a uniformly repopulated valve leaflet construct after 4 weeks of static culture.
Leaflet Interstitial Cells
TLDR
This chapter will focus on studies of atrioventricular and aortic valve LIC in pig, rabbit, hamster, rat, mouse, and human, and the structural and functional characteristics of these cells.
Characterization of Porcine Aortic Valvular Interstitial Cell ‘Calcified’ Nodules
TLDR
It is demonstrated that ‘calcified’ nodules formed from PAVICs grown in OST+TGF-β1 medium do not mineralize after 21 days in culture, but rather they express a myofibroblast-like phenotype and produce a collagen-rich extracellular matrix.
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References

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TLDR
It is suggested that this relative deficiency of in vitro fibronectin synthesis by valvular cells, if paralleled in vivo, may have important implications for the pathogenesis of lesions unique to the cardiac valve.
Interstitial Cells of the Heart Valves Possess Characteristics Similar to Smooth Muscle Cells
TLDR
Observations suggest that VIC may have contractile properties, which can account for a controlled tonus, actively correlated with the cyclically changing forces acting on valves during diastole and systole.
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