Porcine cardiac valvular subendothelial cells in culture: cell isolation and growth characteristics.

  title={Porcine cardiac valvular subendothelial cells in culture: cell isolation and growth characteristics.},
  author={C. M. Johnson and Mark Nils Hanson and S C Helgeson},
  journal={Journal of molecular and cellular cardiology},
  volume={19 12},

Smoothelin-positive cells in human and porcine semilunar valves

The examination of valve interstitial cells confirmed the presence of terminally differentiated, contractile smooth muscle cells in situ and suggested that smooth muscle α-actin and smoothelin interact, as has been previously postulated.

Robust Generation of Quiescent Porcine Valvular Interstitial Cell Cultures

The approach using a fibroblast media formulation with culture on collagen coatings generates quiescent VICs that more accurately mimic a healthy VIC population and thus has the potential to transform the study of the mechanisms of VIC activation and dysfunction involved in the early stages of calcific aortic valve disease.

Fibronectin-based isolation of valve interstitial cell subpopulations: relevance to valve disease.

Myxomatous mitral valves (MVs) contain elevated proportions of unique cell populations such as myofibroblasts. Without a reliable technique to isolate such cell populations, however, it has been

Interstitial cells from the atrial and ventricular sides of the bovine mitral valve respond differently to denuding endocardial injury

In organ culture, interstitial cells from the ventricular side of the mitral valve respond to a denuding endocardial injury by proliferating and migrating onto the adjacent surface whereas interstitial Cells from the atrial side do not.

Characterization of Cell Subpopulations Expressing Progenitor Cell Markers in Porcine Cardiac Valves

Cell profiling based on flow cytometry and functional studies with sorted primary cells provide not only new and quantitative information about the cellular composition of porcine cardiac valves, but also contribute to the understanding of how a subpopulation of valvular cells (ABCG2+ cells) may participate in tissue repair and disease progression.

Serum deprivation improves seeding and repopulation of acellular matrices with valvular interstitial cells.

A reduction of serum content was found to increase significantly the number of adherent cells, as well as induce transfer of VICs from a tissue-culture polystyrene surface to the aScaffold, leading to a uniformly repopulated valve leaflet construct after 4 weeks of static culture.

Leaflet Interstitial Cells

This chapter will focus on studies of atrioventricular and aortic valve LIC in pig, rabbit, hamster, rat, mouse, and human, and the structural and functional characteristics of these cells.

Characterization of Porcine Aortic Valvular Interstitial Cell ‘Calcified’ Nodules

It is demonstrated that ‘calcified’ nodules formed from PAVICs grown in OST+TGF-β1 medium do not mineralize after 21 days in culture, but rather they express a myofibroblast-like phenotype and produce a collagen-rich extracellular matrix.

Substrate properties influence calcification in valvular interstitial cell culture.

Both, the biochemical and mechanical environment of tissue culture has an effect on the spontaneous calcification of VICs, and may also have a profound effect on their molecular properties, as related to an understanding of the disease process in vivo.



Porcine cardiac valvular endothelial cells in culture. A relative deficiency of fibronectin synthesis in vitro.

It is suggested that this relative deficiency of in vitro fibronectin synthesis by valvular cells, if paralleled in vivo, may have important implications for the pathogenesis of lesions unique to the cardiac valve.

Interstitial Cells of the Heart Valves Possess Characteristics Similar to Smooth Muscle Cells

Observations suggest that VIC may have contractile properties, which can account for a controlled tonus, actively correlated with the cyclically changing forces acting on valves during diastole and systole.

Cultured endothelial cells produce a platelet-derived growth factor-like protein.

The platelet-derived growth factor (PDGF) binds specifically to high-affinity receptors on the surface of bovine aortic smooth muscle cells and 3T3 cells. Conditioned medium from cultured bovine

Biochemical properties of the endothelium‐derived growth factor: Comparision to other growth factors

Cultured bovine aortic endothelial cells (BAEC) can be maintained at saturation density for several weeks in the absence of serum. These cells retain viability and normal culture morphology, and

Locally acting growth factors for vascular smooth muscle cells: endogenous synthesis and release from platelets.

Activation of endogenous synthesis of PDGF-c may contribute to the smooth muscle cell proliferation seen in response to vascular injury.


Not only did transformation alter the cellular requirement for PDGF, but infection with SV40 also was demonstrated to alter the plasma requirements necessary for progression, and two plasma-dependent arrest points have been described within this portion of the cell cycle.

Both normal and tumor cells produce basic fibroblast growth factor

We have previously purified from human placenta a basic fibroblast growth factor (FGF)‐like molecule which stimulates the production of plasminogen activator (PA) and collagenase, induces DNA

A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro.

Much of the growth-promoting activity of dialyzed serum is directly or indirectly derived from platelets, which has important implications for the response of arteries to localized injury and provides a key to further understanding of the role of factors derived from blood serum in promoting cell proliferation in vitro.