Population structure and genetic variability within isolates of Grapevine fanleaf virus from a naturally infected vineyard in France: evidence for mixed infection and recombination.

@article{Vigne2004PopulationSA,
  title={Population structure and genetic variability within isolates of Grapevine fanleaf virus from a naturally infected vineyard in France: evidence for mixed infection and recombination.},
  author={Emmanuelle Vigne and Marc Bergdoll and S{\'e}bastien Guyader and Marc Fuchs},
  journal={The Journal of general virology},
  year={2004},
  volume={85 Pt 8},
  pages={2435-45}
}
The nematode-borne Grapevine fanleaf virus, from the genus Nepovirus in the family Comoviridae, causes severe degeneration of grapevines in most vineyards worldwide. We characterized 347 isolates from transgenic and conventional grapevines from two vineyard sites in the Champagne region of France for their molecular variant composition. The population structure and genetic diversity were examined in the coat protein gene by IC-RT-PCR-RFLP analysis with EcoRI and StyI, and nucleotide sequencing… CONTINUE READING

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