Population screening for the common G985 mutation causing medium-chain acyl-CoA dehydrogenase deficiency with Eu-labeled oligonucleotides and the DELFIA system.

@article{Seddon1997PopulationSF,
  title={Population screening for the common G985 mutation causing medium-chain acyl-CoA dehydrogenase deficiency with Eu-labeled oligonucleotides and the DELFIA system.},
  author={Helena Seddon and Gary Gray and Rodney J. Pollitt and Antti Iiti{\"a} and Anne McKenney Green},
  journal={Clinical chemistry},
  year={1997},
  volume={43 3},
  pages={
          436-42
        }
}
We have screened 10171 neonatal blood spots from the Trent and West Midlands regions of the UK for the common G985 mutation to more accurately establish the incidence of medium-chain acyl coenzyme (Co)A dehydrogenase (MCAD) deficiency. We have used a technique involving PCR and Eu-labeled allele-specific oligonucleotides detected by using time-resolved fluorometry on the dissociation-enhanced fluorescence immunoassay (DELFIA) system for the detection of the G985 mutation. We have also evaluated… CONTINUE READING

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