Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study

  title={Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study},
  author={Jan E. Janecka and R. Jackson and Z. Yuquang and Li Diqiang and Bayaraa Munkhtsog and Valerie A. Buckley-Beason and W. J. Murphy},
  journal={Animal Conservation},
The endangered snow leopard Panthera uncia occurs in rugged, high‐altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize moecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard‐specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking… 

Noninvasive genetic population survey of snow leopards (Panthera uncia) in Kangchenjunga conservation area, Shey Phoksundo National Park and surrounding buffer zones of Nepal

Noninvasive genetic techniques were utilized to confirm the occurrence of snow leopards in both study areas and determine the minimum number present, which can be used to design more in-depth population surveys that will enable estimation ofSnow leopard population abundance at these sites.

A pilot study—genetic diversity and population structure of snow leopards of Gilgit-Baltistan, Pakistan, using molecular techniques

The low gene flow rate among the population results in low genetic diversity causing decreased diversification, which affects the adaptability to climatic changes, thus ultimately resulting in decreased population size of the species.

Multipronged strategy including genetic analysis for assessing conservation options for the snow leopard in the central Himalaya

Abstract The snow leopard (Panthera uncia) is an endangered carnivore of southern and central Asia. Approximate 10% of the global population occurs in the Himalayan region of Nepal. The snow leopard

Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples

Primers for 9 microsatellites loci previously isolated in the domestic cat are designed specifically for snow leopard studies using noninvasive samples to facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.

Comparison of noninvasive genetic and camera-trapping techniques for surveying snow leopards

The endangered snow leopard (Panthera uncia) is widely but sparsely distributed throughout the mountainous regions of central Asia. Detailed information on the status and abundance of the snow

Population genetics of the snow leopards (Panthera uncia) from the Western Himalayas, India

The snow leopard ( Panthera uncia ), one of the most threatened felids distributed along the high-altitude mountains in the Himalayas, has experienced a steady population decline in most of its

Range-Wide Snow Leopard Phylogeography Supports Three Subspecies

The first range-wide genetic assessment ofSnow leopards based on noninvasive scat surveys provides a foundation for global conservation of snow leopard subspecies, and set the stage for in-depth landscape genetics and genomic studies.

Modelling potential habitat for snow leopards (Panthera uncia) in Ladakh, India

This is the first study to model snow leopard distribution both in India and utilising direct observation data, and suggested that elevation and ruggedness are the two most influential environmental variables forSnow leopard habitat suitability.

Camera-trapping snow leopards in the Tost Uul region of Mongolia

The snow leopard’s reclusive behaviour, generally low population density, fragmented distribution and rugged mountain habitat make it exceptionally hard to enumerate. Since conservation success

Snow leopard (Panthera uncia) surveys in the Western Himalayas, India

We conducted surveys above 3000 m elevation in eight protected areas of Uttarakhand and Himachal Pradesh. These surveys provide new information on snow leopard in Uttarakhand on the basis of indirect



A select panel of polymorphic microsatellite loci for individual identification of snow leopards (Panthera uncia)

To develop molecular genetic tools for individual identification of hair and faecal samples, 50 microsatellite loci developed for the domestic cat in 19 captive snow leopards were screened.

Recovery of snow leopard Uncia uncia in Sagarmatha (Mount Everest) National Park, Nepal

From September to November 2004 we conducted surveys of snow leopard Uncia uncia signs in three major valleys in Sagarmatha (Mount Everest) National Park in Nepal using the Snow Leopard Information

Estimating Snow Leopard Population Abundance Using Photography and Capture–Recapture Techniques

Abstract Conservation and management of snow leopards (Uncia uncia) has largely relied on anecdotal evidence and presence–absence data due to their cryptic nature and the difficult terrain they

Dietary separation of sympatric carnivores identified by molecular analysis of scats

Analysis of diets of four sympatric carnivores in the flooding savannas of western Venezuela by analysing predator DNA and prey remains in faeces shows that puma and jaguar scats overlap in size, as do those of puma, ocelot and fox.

The status of the snow leopard in Pakistan and its conflict with local farmers

The long-term future of the snow leopard is under threat mainly due to retaliatory killings by farmers, and poaching for pelts and other body parts, in Baltistan and throughout its range within Pakistan's borders.

Molecular Evidence for Species-Level Distinctions in Clouded Leopards

Landscape ecology of eastern coyotes based on large-scale estimates of abundance.

  • R. KaysM. GompperJ. Ray
  • Environmental Science
    Ecological applications : a publication of the Ecological Society of America
  • 2008
Habitat models predicting coyote abundance using multi-scale vegetation and landscape data and an information-theoretic model selection approach reject the hypothesis that eastern forests are unsuitable habitat for coyotes and support the need for a nuanced view of how eastern coyotes use forested habitats.

Importance of a pilot study for non-invasive genetic sampling: genotyping errors and population size estimation in red deer

The importance of conducting a pilot study when using non-invasive sampling to study threatened or managed populations is illustrated, with results suggest the authors must conduct 6 PCR amplifications per sample (per locus) to achieve approximately 97% correct genotypes.