• Biology
  • Published 2016

Pooling of faecal samples for quantitative virus diagnostics by real-time PCR

@inproceedings{Hartby2016PoolingOF,
  title={Pooling of faecal samples for quantitative virus diagnostics by real-time PCR},
  author={Christina Marie Hartby and Malene Rask Andersen and Lise Kirstine Kvisgaard and Mariann Chri{\'e}l and Lars Erik Larsen and Charlotte Kristiane Hjulsager},
  year={2016}
}
Fecal samples from 21 healthy and 14 diarrheic foxes as well as six healthy and 11 diarrheic mink from ten farms were collected in September 2015. The fecal microbiota was studied using a next generation sequencing (NSG) method. For the comparison of bacterial flora, the V3 and V4 regions of 16S RNA gene were sequenced. For the fecal virome analysis a random amplification followed by NGS was conducted. The metagenomic analysis suggested that foxes have more diverse bacterial microbiota than the… CONTINUE READING

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