Pontine microinjection of carbachol does not reliably enhance paradoxical sleep in rats.

@article{Deurveilher1997PontineMO,
  title={Pontine microinjection of carbachol does not reliably enhance paradoxical sleep in rats.},
  author={Samuel Deurveilher and Bernard Hars and Elizabeth Hennevin},
  journal={Sleep},
  year={1997},
  volume={20 8},
  pages={593-607}
}
It has been repeatedly shown in cats that acute administration of carbachol into the pontine reticular formation (PRF) readily evokes a state that closely mimics natural paradoxical sleep (PS). Surprisingly, there are few corresponding studies in rats. In order to further characterize the effects of pontine carbachol in rats, 151 injections of different doses (from 3 micrograms to 0.005 microgram in 0.1 microliter saline) of carbachol were made at different sites within the PRF of 70 rats… CONTINUE READING

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