Pom1p, a fission yeast protein kinase that provides positional information for both polarized growth and cytokinesis.

@article{Bhler1998Pom1pAF,
  title={Pom1p, a fission yeast protein kinase that provides positional information for both polarized growth and cytokinesis.},
  author={J{\"u}rg B{\"a}hler and John R. Pringle},
  journal={Genes \& development},
  year={1998},
  volume={12 9},
  pages={
          1356-70
        }
}
Schizosaccharomyces pombe cells have a well-defined pattern of polarized growth at the cell ends during interphase and divide symmetrically into two equal-sized daughter cells. We identified a gene, pom1, that provides positional information for both growth and division in S. pombe. pom1 mutants form functioning growth zones and division septa but show several abnormalities: (1) After division, cells initiate growth with equal frequencies from either the old or the new end; (2) most cells never… Expand
Fission yeast Pom1p kinase activity is cell cycle regulated and essential for cellular symmetry during growth and division
TLDR
It is shown that Pom1p kinase activity is cell cycle regulated in correlation with the state of cellular symmetry: the activity is high during symmetrical growth and division, but lower when cells grow at just one end, and there may be related roles of homologous protein kinases ubiquitously present in all eukaryotes. Expand
Tea3p Is a Cell End Marker Activating Polarized Growth in Schizosaccharomyces pombe
TLDR
It is proposed that Tea3p is a novel cell end marker required specifically to activate polarized cell growth at the second end during NETO, which is required for efficient NETO and for the proper placement of the septum. Expand
The Cell-End Factor Pom1p Inhibits Mid1p in Specification of the Cell Division Plane in Fission Yeast
TLDR
Computational and experimental results support a model in which both positive cues from the medial nucleus and negative cue from the cell tips specify the position of the division plane, and evidence that pom1p, a DYRK-family protein kinase that forms a concentration gradient emanating from the nongrowing cell end, inhibits mid1p is provided. Expand
Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeast
TLDR
These studies delineate a pathway for how microtubule plus ends function to polarize the actin cytoskeleton through actin-associated polarity factors. Expand
Role of Polo Kinase and Mid1p in Determining the Site of Cell Division in Fission Yeast
TLDR
Genetic and two-hybrid analyses suggest that Plo1p and Mid1p act in a common pathway distinct from that involving Pom1p, and indicate that Pla1p plays a role in the positioning of division sites by regulating Mid1 p. Expand
Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe.
TLDR
Electron microscopic observation of mutant cells indicated that the most severe defect is found in eng1Delta agn1Delta cells, lacking the Eng1p endo-beta-1,3-glucanase and the Agn1pendo-alpha-glUCanase, which suggests that these two proteins are the main activities required for cell separation to be completed. Expand
DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division
TLDR
It is established that Pom1’s kinase activity prevents septation at cell tips even if Mid1 is absent or mislocalized, and inhibition of Cdc15-scaffolded septum formation at cell poles is a key Pom1 mechanism that ensures medial division. Expand
Reorganization of the Growth Pattern of Schizosaccharomyces pombe in Invasive Filament Formation
TLDR
This work has found striking alterations to the behavior of some of the key regulatory proteins in the organization and control of polarized growth through the cell cycle of Schizosaccharomyces pombe, a single-celled eukaryote. Expand
A checkpoint that monitors cytokinesis in Schizosaccharomyces pombe.
TLDR
It is shown that the inability of cps1-191 mutants to proceed through multiple mitotic cycles is not related to a defect in cell growth, and the failure to complete some aspect of cytokinesis may prevent the G(2)/M transition of the two interphase-G(2) nuclei. Expand
Function of rax2p in the polarized growth of fission yeast.
TLDR
Observations suggest that rax2p controls the cell polarity of fission yeast during vegetative growth by regulating for3p localization. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 59 REFERENCES
The Schizosaccharomyces pombe cdc14 gene is required for septum formation and can also inhibit nuclear division.
TLDR
The cloning of the cdc14 gene and the identification of its product shows that the gene is essential for septum formation and completion of the cell-division cycle, consistent with the idea that p28cdc14 may play a role in both the initiation of mitosis and septal formation and, by doing so, be part of the mechanism that coordinates these two cell-cycle events. Expand
The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis
TLDR
It is proposed that Cdc3-profilin plays an essential role in cytokinesis by catalyzing the formation of the F-actin contractile ring, and that it is essential for F- actin ring formation. Expand
Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis
TLDR
This work cloned and characterized the cdc4 gene and found that Cdc4 protein forms a complex with a 200-kD protein which can be cross-linked to UTP, a property common to myosin heavy chains, and suggests that CDC4p may be a novel myosIn light chain. Expand
The dmf1/mid1 gene is essential for correct positioning of the division septum in fission yeast.
TLDR
Data implicate Dmf1p as an important element in assuring correct placement of the division septum in Schizosaccharomyces pombe cells. Expand
Genetic analysis of cell morphogenesis in fission yeast‐‐a role for casein kinase II in the establishment of polarized growth.
We have initiated a study to identify genes regulating cell morphogenesis in the fission yeast Schizosaccharomyces pombe. Five genes have been identified, orb1‐orb5, whose mutation gives rise toExpand
Growth Polarity and Cytokinesis in Fission Yeast: The Role of the Cytoskeleton
TLDR
The distribution of F-actin in the fission yeast Schizosaccharomyces pombe was investigated by fluorescence microscopy using rhodamine-conjugated phalloidin and a system of cytoplasmic microtubules extending between the cell ends was revealed. Expand
Growth in cell length in the fission yeast Schizosaccharomyces pombe.
TLDR
It is concluded that NETO only happens if: the cell length is greater than a critical value of 9.0-9.5 microns and the cell has traversed the first 0.3-0.35 of the cycle and passed early G2, in which cell size is also reduced. Expand
The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe.
TLDR
The unique properties of various cell division cycle mutants are used to investigate further the function of these different microtubule arrays and their temporal and positional control. Expand
Fission yeast cell morphogenesis: identification of new genes and analysis of their role during the cell cycle
To identify new genes involved in the control of cell morphogenesis in the fission yeast Schizosaccharomyces pombe we have visually screened for temperature-sensitive mutants that show defects inExpand
The spindle pole body of Schizosaccharomyces pombe enters and leaves the nuclear envelope as the cell cycle proceeds.
TLDR
Serial sections demonstrate that some interphase microtubules (MTs) end on or very near to the SPB, and apparently, the MT initiation activities of gamma-tubulin in S. pombe are regulated. Expand
...
1
2
3
4
5
...