Polysulfated carbohydrates analyzed as ion-paired complexes with basic peptides and proteins using electrospray negative ionization mass spectrometry.

@article{Siegel1997PolysulfatedCA,
  title={Polysulfated carbohydrates analyzed as ion-paired complexes with basic peptides and proteins using electrospray negative ionization mass spectrometry.},
  author={Marshall M. Siegel and Keiko Tabei and Michael Z. Kagan and Iontcho Radoslavov Vlahov and Ronald E. Hileman and Robert J. Linhardt},
  journal={Journal of mass spectrometry : JMS},
  year={1997},
  volume={32 7},
  pages={
          760-72
        }
}
Electrospray ionization mass spectrometry was used in the negative ion mode to analyze complexes of sucrose octasulfate, sucrose heptasulfate and sulfated alpha-, beta- and gamma-cyclodextrins with synthetically prepared basic peptides, the basic protein ubiquitin and polyamines. The spectra presented demonstrate that complexes with these basic molecules facilitate the analysis of these polysulfated oligosaccharides. Stable (1:1) complexes result from the ion pairing between the protonated… Expand
Liquid chromatography-electrospray mass spectrometry as a tool for the analysis of sulfated oligosaccharides from mucin glycoproteins.
TLDR
The LC-ESI-MS approach should be a valuable tool for characterization of mucin glycosylation and alterations during pathological conditions. Expand
Matrix-assisted laser desorption/ionization mass spectrometric analysis of polysulfated-derived oligosaccharides using pyrenemethylguanidine
TLDR
The present report demonstrates how MALDI-MS can be used to derive structural and compositional information from pure and mixed fractions of polysulfated oligosaccharides and develops a software tool for the rapid and automatic structural elucidation of carrageenans based on the mass spectra obtained. Expand
Competing fragmentation processes in tandem mass spectra of heparin-like glycosaminoglycans
TLDR
Reduction of charge-charge repulsions, such as is achieved by pairing the HLGAG ions with metal cations, is necessary in order to minimize the abundances of ions derived via fragmentation processes that compete with glycosidic bond cleavages. Expand
Liquid Chromatography/Mass Spectrometry Sequencing Approach for Highly Sulfated Heparin-derived Oligosaccharides*
TLDR
UV in combination with MS detection led to the identification of oligosaccharides arising from the original non-reducing end (NRE) of the heparin chain, and high resolution mass spectrometry afforded elemental composition of many known and previously unknown heparIn-derived oligosACcharides. Expand
On-line selective enrichment and ion-pair reaction for structural determination of sulfated glycopeptides by capillary electrophoresis-mass spectrometry.
TLDR
A new capillary electrophoresis-mass spectrometry (CE-MS)-based technique for analyzing sulfated glycopeptides is described, affording approximately 500-fold sensitivity enhancement and structural information was successfully obtained via on-line ion-pair complexation reaction. Expand
A novel mass spectrometric method to distinguish isobaric monosaccharides that are phosphorylated or sulfated using ion-pairing reagents
TLDR
This is the first study demonstrating that ion-pairing can be used for the detection of phosphorylated compounds and the firstStudy to use ion- Pairing in conjunction with MS/MS to obtain structural information about the analytes. Expand
Distinguishing phosphorylation and sulfation in carbohydrates and glycoproteins using ion-pairing and mass spectrometry
TLDR
It is demonstrated that these two groups can be discriminated by using ion-pairing in conjunction with MS/MS experiments and the characteristic product ions are used to successfully identify the phosphorylation and sulfation present in mono-, disaccharides, and the highly sulfated glycoprotein, ovine luteinizing hormone. Expand
Enhanced detection of sulfated glycosylation sites in glycoproteins
TLDR
A method that enhances the mass spectral signal of mono- and disulfated glycopeptides, present in glycoproteins that contain many other nonsulfated glycoforms, is demonstrated and the ion-pairing technique is useful in obtaining structural information about the sulfated species. Expand
Isolation and characterization of β‐cyclodextrin sulfates by preparative gradient polyacrylamide gel electrophoresis, capillary electrophoresis and electrospray ionization—mass spectrometry
A β‐cyclodextrin sulfate mixture has been fractionated using discontinuous gradient polyacrylamide gel electrophoresis. Semidry electrotransfer of the sample onto a positively charged nylon membraneExpand
Determination of the structure of oligosaccharides prepared from acharan sulfate.
TLDR
Results suggest the presence of minor sequence variants in acharan sulfate containing unsulfated iduronic acid having the structure of 4-alpha-D-GlcNpAc(1-->4)-alpha-L-IdoAp2S(1-->) and electrospray ionization mass spectrometry identified two series of oligosaccharides. Expand
...
1
2
3
...