Polystyrene nanoparticle trafficking across alveolar epithelium.

  title={Polystyrene nanoparticle trafficking across alveolar epithelium.},
  author={Nazanin R Yacobi and Lucas Demaio and Jiansong Xie and Sarah F Hamm-Alvarez and Zea Borok and Kwang-Jin Kim and Edward D Crandall},
  journal={Nanomedicine : nanotechnology, biology, and medicine},
  volume={4 2},
We investigated trafficking of polystyrene nanoparticles (PNP; 20 and 100 nm; carboxylate, sulfate, or aldehyde-sulfate modified [negatively charged] and amidine-modified [positively charged]) across rat alveolar epithelial cell monolayers (RAECM). Apical-to-basolateral fluxes of nanoparticles were estimated as functions of apical PNP concentration ([PNP]) and temperature. Uptake of nanoparticles into RAECM was determined using confocal microscopy. Fluxes increased as charge density became less… CONTINUE READING