Polysaccharide production by a reduced pigmentation mutant of the fungus Aureobasidium pullulans

  title={Polysaccharide production by a reduced pigmentation mutant of the fungus Aureobasidium pullulans},
  author={Thomas P. West and B. Reed-Hamer},
  journal={Fems Microbiology Letters},
A reduced pigmentation mutant was isolated from Aureobasidium pullulans ATCC 42023 by chemical mutagenesis and was subsequently characterized. The pigment melanin was present not only in A. pullulans cells but also contaminated the elaborated polysaccharide and thus, was measured in both fractions. Cellular and polysaccharide melanin levels of the mutant strain were at least 11-fold and 18-fold reduced, respectivelu, compared toits parent strain after 7 days of growth at 30°C whether sucrose or… 
Elevated polysaccharide production by mutants of the fungus Aureobasidium pullulans
The dry weights of the mutant cells were found not to differ greatly from those of the parent cells whether corn syrup or sucrose served as the carbon source, and the pullulan content of the polysaccharide elaborated by the parent was higher than either mutant.
Enhanced production of pigment-free pullulan by a morphogenetically arrested Aureobasidium pullulans (ATCC 42023) in a two-stage fermentation with shift from soy bean oil to sucrose
Morphogenesis from the yeast form of the microorganism to chlamydospores was still restrained and no pigment was formed in the culture during the production stage, and a pigment-free polysaccharide was recovered from the supernatant of the broth after solvent precipitation.
Fungal production of the polysaccharide pullulan from a plant hydrolysate
  • T. West
  • Medicine, Biology
    Zeitschrift fur Naturforschung. C, Journal of biosciences
  • 2017
Unlike what has been observed for other biomass substrates, a polysaccharide with a high pullulan content can be produced at a relatively high yield by a fungus grown on a grass hydrolysate indicating thatpullulan could be produced using a biomass-based process.
Pullulan production by Aureobasidium pullulans grown on corn steep solids as a nitrogen source
The pullulan content of the polysaccharide elaborated by ammonium sulphate-grown cells on day 7 was generally higher than the pullulans content of poly Saccharide produced by corn steep solids- grown cells, although the pullULan content was found to increase as the corn syrup concentration increased.
Effect of nitrogen source on pullulan production by Aureobasidium pullulans grown in a batch bioreactor.
The pullulan content of the polysaccharide elaborated by ammonium sulphate-grown cells on day 7 decreased as the corn syrup level rose in the medium while the pullulans content of polysacCharide produced by cells grown on corn steep liquor or soytone generally increased.
Effect of carbon source on polysaccharide production by alginate‐entrapped Aureobasidium pullulans ATCC 42023 cells
  • T. West
  • Engineering, Biology
    Journal of basic microbiology
  • 2011
The production of the exopolysaccharide pullulan using entrapped cells of the fungus Aureobasidium pullulans ATCC 42023 was investigated relative to carbon source and independent of carbon source, the entrapping fungal cells exhibited a higher yield during the initial cycle than the second production cycle.
Pullulan production by Aureobasidium pullulans cells immobilized on ECTEOLA-cellulose
  • T. West
  • Biology, Engineering
    Annals of Microbiology
  • 2010
It was found that the cells immobilized on the ECTEOLA-cellulose at pH 2.0 produced higher pullulan levels than those cells immobilizing at pH 3.0, which was not statistically significant.
Production of pigment-free pullulan by swollen cell in Aureobasidium pullulans NG which cell differentiation was affected by pH and nutrition
Controlling pH of fermentation is an effective and convenient method to harvest SC for melanin-free pullulan production.


A comparison of polyssacharides from strains of Aureobasidium pullulans
A “color variant” strain of Aureobasidium, NRRL Y-12974, possibly representing a distinct species, produced a polysaccharide of intermediate molecular weight which was stable during storage at 4°C, heating to 100°C and high shearing action and contained the least contaminating melanin pigment.
Effect of pH on the batch fermentation of pullulan from sucrose medium
The bistaged pH process for the production of pullulan enhanced the polysaccharide concentration in the medium, influenced the rheological properties of the fermentation broth, and has a potential of operation under nonsterile and nonaseptic conditions.
Polysaccharide produced by the genus Pullularia. I. Production of polysaccharide by growing cells.
A dextranlike polysaccharide was found to be produced on substrates of sucrose, maltose, glucose, and fructose by growing cells of various strains of the genus Pullularia. The polysaccharide,
Effect of pH on Pullulan Elaboration by Aureobasidium pullulans S-1
The capability of Aureobasidium pullulans S-1 to produce pullulan varied considerably with the initial pH; that is, pullulan was hardly elaborated with initial pH 2 ~ 2.5, and the organism converted
Utilization of carbon sources by Pullularia pullulans for the elaboration of extracellular polysaccharides.
The yeastlike fungus Pullularia pullulans utilizes simple mono- and disaccharides both in the production of cell mass and the elaboration of extracellular polysaccharide. The utilization pattern of
Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans
Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethyl ammonium hydroxide into soluble and insoluble fractions, and the struc...
The production of polysaccharides by fungi active in the decomposition of wood and forest litter.
  • B. Bernier
  • Environmental Science
    Canadian journal of microbiology
  • 1958
Four fungi known to be active in the decomposition of wood and forest litter were grown on a liquid medium containing salts and glucose and also on aqueous extracts of a variety of fresh leaves and...