Polysaccharide analysis using carbohydrate gel electrophoresis: a method to study plant cell wall polysaccharides and polysaccharide hydrolases.

@article{Goubet2002PolysaccharideAU,
  title={Polysaccharide analysis using carbohydrate gel electrophoresis: a method to study plant cell wall polysaccharides and polysaccharide hydrolases.},
  author={Florence Goubet and Peter Jackson and Michael J. Deery and Paul Dupree},
  journal={Analytical biochemistry},
  year={2002},
  volume={300 1},
  pages={53-68}
}
A method to characterize plant cell wall polysaccharides is presented. The complexity of the polymer structures and the large number of different charged and uncharged monosaccharides that make up plant polysaccharides have previously made analysis technically demanding and laborious. Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of reducing ends of sugars and oligosaccharides with a fluorophore, followed by electrophoresis under optimized… CONTINUE READING

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