Polyreactive binding of antibodies generated by polyclonal B cell activation. I. Polyreactivity could be caused by differential glycosylation of immunoglobulins.

@article{Fernndez1997PolyreactiveBO,
  title={Polyreactive binding of antibodies generated by polyclonal B cell activation. I. Polyreactivity could be caused by differential glycosylation of immunoglobulins.},
  author={Carmen Fern{\'a}ndez and Marta E. Alarc{\'o}n-Riquelme and Manuchehr Abedi-Valugerdi and Eva Sverremark and V{\'i}ctor Cort{\'e}s},
  journal={Scandinavian journal of immunology},
  year={1997},
  volume={45 3},
  pages={231-9}
}
The aim of this study was to gain knowledge of the pre-immune repertoire with reactivity directed to the carbohydrate antigen dextran B512 (Dx). Polyclonal activation of spleen cells in mice has been estimated to be a method of revealing the available repertoire. Hybridoma cell lines derived from lipopolysaccharide (LPS)-stimulated C57BL/6 spleen cells were screened for reactivity with Dx and with the non-related protein bovine serum albumin (BSA). Despite the lack of structural similarity… CONTINUE READING