Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov..

@article{Logan2002PolyphasicIO,
  title={Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov..},
  author={Niall A. Logan and Gillian Forsyth and Liesbeth Lebbe and Johan Goris and Marc Heyndrickx and An Balcaen and An Verhelst and Enevold Falsen and Åsa Ljungh and H B Hansson and Paul de Vos},
  journal={International journal of systematic and evolutionary microbiology},
  year={2002},
  volume={52 Pt 3},
  pages={
          953-66
        }
}
Thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rDNA restriction analysis (ARDRA), 16S rDNA sequencing and DNA-DNA reassociation, and phenotypically by using fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins, API Biotype 100 assimilation tests and 16 other routine phenotypic tests. Three isolates were identified as strains of… 

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References

SHOWING 1-10 OF 23 REFERENCES
Proposal of Bacillus reuszeri sp. nov., Bacillus formosus sp. nov., nom. rev., and Bacillus borstelensis sp. nov., nom. rev.
TLDR
The S- Layer proteins found in the three new species were immunologically and genetically similar to the S-layer proteins found previously in B. brevis and the recently described species Bacillus agri, Bacillus centrosporus, Baciller migulanus, Bacillin choshinensis, and Bacillus parabrevis.
DNA relatedness of Bacillus brevis Migula 1900 strains and proposal of Bacillus agri sp. nov., nom. rev., and Bacillus centrosporus sp. nov., nom. rev.
TLDR
The phenotypic heterogeneity of B. brevis sensu lato results from the inclusion of genetically unrelated organisms in the taxon and not from the inherent variability of genetically related strains.
Characterization of Bacillus brevis with descriptions of Bacillus migulanus sp. nov., Bacillus choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov.
TLDR
Thirty-five Bacillus brevis strains obtained from culture collections, including protein-producing isolates, were taxonomically studied by using numerical analysis, DNA base composition, and DNA-DNA hybridization to identify species of the genus Bacillus.
Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.
TLDR
16S rRNA gene sequences of the type strains of 11 species belonging to the Bacillus brevis and Bacillus aneurinolyticus groups were determined and two new genera were proposed, which were phylogenetically distinct from other Bacillus, Amphibacillus, Sporolactobacilli, Paenibacillin, and Alicyclobacillus species.
Virgibacillus : a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus
TLDR
The species Virgibacillus pantothenticus and related organisms comprising this new genus can be distinguished fromMembers of Bacillus rRNA group 1 (Bacillus sensu stricto), and from members of Paenibacillin and other aerobic endospore-forming bacteria by routine phenotypic tests.
Phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera Caryophanon, Exiguobacterium, Kurthia, and Planococcus.
TLDR
A comparative analysis of the sequence data revealed that the round-spore-forming group 2 bacilli, together with some asporogenous taxa, form a phylogenetically distinct cluster that is only remotely related to Bacillus subtilis, the type species of the genus Bacillus.
Differentiation of Xanthomonas campestris pv. Citri Strains by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis of Proteins, Fatty Acid Analysis, and DNA-DNA Hybridization
A total of 61 strains, including members of all five currently described pathogenicity groups of Xanthomonas campestris pv. citri (groups A, B, C, D, and E) and representing a broad geographical
Bacillus species of medical and veterinary importance.
TLDR
The increasing frequency with which opportunist infections are encountered is in part due to host predisposition by suppressed or compromised immunity, metabolic disorder, and malignant disease, or to exposure by surgical and clinical procedures, accidental trauma and drug abuse; it is also associated with advances in bacteriological technique and interpretation.
Phylogenetic analysis of the genera Planococcus, Marinococcus and Sporosarcina and their relationships to members of the genus Bacillus.
TLDR
A phylogenetic analysis based on 16S rRNA was performed on the genera Planococcus, Marinitiscus, Sporosarcina and endospore-forming rods, showing that Marinococcus halophilus exhibited low levels of relatedness to all reference species examined and formed a distinct line of descent.
...
...