Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA

  title={Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA},
  author={Joan A. Steitz},
  • J. Steitz
  • Published 6 December 1969
  • Biology, Chemistry, Physics
  • Nature
The initiator regions of the three cistrons of R17 bacteriophage RNA have been isolated and sequenced. All three regions contain a UGA triplet as well as the expected AUG and two contain the sequence GGUUUGA. The initiator regions thus represent untranslated sequences between termination and initiation codons. 
Nucleotide Sequence from the Polypeptide Chain Termination Region of the Coat Protein Cistron in Bacteriophage R17 RNA
The nucleotide sequence of a fragment of R17 RNA, which corresponds to the last six amino-acids of the bacteriophage coat protein, and extends on the 3′ side of the coat protein cistron for
Bacteriophage Ms2 RNA: nucleotide sequence of the end of the a protein gene and the intercistronic region.
The sequence of an RNA segment of 160 nucleotides preceding the coat protein initiator AUG has been established. It contains the genetic information for the last 45 amino-acids of the A or maturation
Determinant of cistron specificity in bacterial ribosomes
Complementarity relationships between this sequence and a purine-rich tract in the ribosome binding site of different bacterial mRNAs suggest that the 3′-end of 16S RNA determines the intrinsic capacity of ribosomes to translate a particular cistron.
Ribosome Binding Site of Qβ RNA Polymerase Cistron
IN conditions of polypeptide chain initiation, 70S ribosomes bind to intact bacteriophage RNA predominantly at the initiation region of the coat protein cistron1,2. The binding sites of the A protein
Untranslated Nucleotide Sequence at the 5′-End of R 17 Bacteriophage RNA
The 5′-terminal sequence of 74 nucleotides reported here does not seem \[tmacr]o be involved in the synthesis of phage specific proteins but is probably concerned in the replication of R17 RNA. It is
Isolation and Sequence Analysis of a Ribosome-protected Fragment from Bacteriophage φX 174 DNA
The DNA sequence corresponding to the gene G spike protein initiation site has been determined by analysis of endonuclease IV and ‘Streptodornase’ digests. The sequence has many features in common
Gene Order in the Bacteriophage R17 RNA : 5′–;A Protein–Coat Protein–Synthetase–3′
The sequencing of the ribosome binding sites and parts of the coat protein cistron has enabled the order of the three R17 genes to be established by direct chemical means. Translation may be


Nucleotide Sequence from the Coat Protein Cistron of R17 Bacteriophage RNA
The sequence of fifty-seven nucleotides in the coat protein cistron of phage R17 RNA directly confirms the genetic code, shows that the code used by the phage is degenerate and suggests that highly
Binding Site on R17 RNA for Coat Protein
A 60 per cent fragment of R17 RNA from the 3′ terminated end contains a binding site which specifically binds one molecule of R17 coat protein. This may be the binding site involved in the inhibition
Sequences at the 5′-Terminus of Bacteriophage Qβ RNA
Two beginning sequences have been identified in the RNA of bacteriophage Qβ and both were consistently found in nearly equal amounts.
Bacteriophage f2 RNA: Control of Translation and Gene Order
The use of a novel protein fingerprinting technique has shown that, in a cell-free system, the three genes of bacteriophage f2 can be translated independently. A crude genetic map of f2 RNA was
Direct Translation of a Circular Messenger DNA
Formyl-methionyl-tRNA is bound to ribosomes by single stranded circular DNA of the phage fd. Addition of neomycin B to this complex permits direct translation of this DNA into protein, which implies
GTP-stimulated Binding of Initiator-tRNA to Ribosomes directed by f2 Bacteriophage RNA
Binding of formyl methionyl-tRNA to ribosomes, directed by RNA extracted from the f2 bacteriophage, is dependent upon initiation factors, GTP and the presence of the formyl group, but is independent of initiating factors, amino acyl- tRNA and GTP.
The 5'-terminus of bacteriophage R17 RNA: pppGp.
  • R. Roblin
  • Biology
    Journal of molecular biology
  • 1968