Polymorphonuclear neutrophil leukocyte function in clinical bovine patients and in cows with or withoutStaphylococcus aureus mastitis

A fluorochrome microassay was used to investigate peripheral blood polymorphonuclear leukocyte (PMNL) function in cattle. Glass-adherent PMNL were reacted withStaphylococcus aureus princubated in 20% bovine serum for 30, 60 and 90 min. Coverslips were stained with acridine organge (AO) followed by crystal violet to quench extracellular bacterial… (More)