Polymorphism of the leghemoglobin gene in Phaseolus demonstrated by polymerase chain reaction amplification

  title={Polymorphism of the leghemoglobin gene in Phaseolus demonstrated by polymerase chain reaction amplification},
  author={Paul W. Skroch and Raymond C. Dobert and Eric W. Triplett and James Nienhuis},
SummaryGenetic variance within Phaseolus vulgaris or among Phaseolus species for leghemoglobin composition may be useful in breeding for enhanced nitrogen fixation. Using primers constructed from conserved regions of the leghemoglobin gene, polymerase chain reaction was used to specifically amplify the Lba gene in total DNA samples from 17 lines and 10 species of Phaseolus. These primers are 100% homologous with the 5′- and 3′-ends of the leghemoglobin-encoding genes Lba of Phaseolus vulgaris… 
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Molecular markers for genebank management
IPGRI Technical Bulletins are published by the International Plant Genetic Resources Institute with the intention of putting forward definitive recommendations for techniques in genetic resources.


Leghemoglobin variability in the genus Phaseolus
Interspecific hybrids were produced to transfer existing leghemoglobin variability into the P. vulgaris background and leghemoglobins were also shown to be inherited codominantly in interspecific hybrids.
Structure and chromosomal arrangement of leghemoglobin genes in kidney bean suggest divergence in soybean leghemoglobin gene loci following tetraploidization
The presence in the kidney bean genome of four leghemoglobin genes suggests that tandem duplications of a single primordial plant globin gene had occurred to generate four legemoglobin genes in an ‘Lb‐locus’ before Glycine and Phaseolus species diverged.
Predicting interspecific compatibilities in beans (Phaseolus) by seed protein electrophoresis
The resultant dendrogram generally agreed with previous research on interspecific relationships in Phaseolus based on morphological characteristics, and also generally agrees with current information on inter specific similarities based on hybridization studies.
Mitochondrial genome size variation and restriction fragment length polymorphisms in threePhaseolus species
Restriction patterns of mitochondrial DNA from threePhaseolus species were examined to estimate their relative genome sizes and to determine the level of interspecific variability and relatedness and it was demonstrated that P. vulgaris and P. coccineus are more related to each other than either is toP.
Conservation of leghemoglobin heterogeneity and structures in cultivated and wild soybean
The conservation of both leghemoglobin heterogeneity and also all four major Leghemoglobin structures provides strong circumstantial evidence that leghemoglobins heterogeneity is functional.
Analyses of Phaseolus vulgaris L. and P. coccineus Lam. hybrids by RFLP: preferential transmission of P. vulgaris alleles
Restriction fragment length polymorphism (RFLP) was determined among P. vulgaris genotypes and Phaseolus species using 19 probes and statistical analyses indicated gametic selection with preferential transmission of the P. Vulgaris alleles may account for the selective recovery of P. coccineus hybrids.
Expression of nodule-specific genes in Phaseolus vulgaris L.
The modified expression of main nodulins observed between these nodules indicates that different levels and/or factors associated with their regulation are involved, and the intracellular infection by Rhizobium as a decisive step in the induction of some P. vulgaris nodULins is discussed.
Heterosis for symbiotic nitrogen fixation in Phaseolus interspecific hybrids
Interspecific hybrids of Phasolus vulgaris × Phaseolus acutifolius and Phaseolu vulgaris–Phaseolus filiformis had significantly (P<0.01) higher nitrogen fixation rates and accumulated more total nitrogen, than either parent, and the interspecific hybrids also showed more complex leghemoglobin profiles than either parents.