Polymorphism of insertion sites of Ty1-copia class retrotransposons and its use for linkage and diversity analysis in pea

  title={Polymorphism of insertion sites of Ty1-copia class retrotransposons and its use for linkage and diversity analysis in pea},
  author={T. H. Noel Ellis and S. J. Poyser and Maggie R. Knox and Alexander V. Vershinin and Mike J Ambrose},
  journal={Molecular and General Genetics MGG},
A sample of 15 cultivars and 56 Pisum accessions from the JIC germplasm core collection has been studied using a modification of the SSAP (sequence-specific amplification polymorphisms) technique; the specific primer was designed to correspond to the polypurine tract (PPT) of PDR1, a Ty1-copia group retrotransposon of pea. Most of these SSAP products were shown to be PDR1 derived. The PDR1 SSAP markers are more informative than previously studied AFLP or RFLP markers and are distributed… CONTINUE READING
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