Polymorphic microsatellites for the study of newly established populations of the gastropod Cyclope neritea

  title={Polymorphic microsatellites for the study of newly established populations of the gastropod Cyclope neritea},
  author={Benoit Simon-Bouhet and Claire Daguin and Pascale Garcia-Meunier and Fr{\'e}d{\'e}rique Viard},
  journal={Molecular Ecology Notes},
Both human-mediated introductions and climatic changes may promote the settlement of species in new areas outside of their natural geographical range. To investigate the settlement of recently established populations of the neogastropod Cyclope neritea , we developed eight microsatellite markers. Their usefulness was studied in two native populations previously found to be monomorphic with mitochondrial markers. The eight loci were found to be polymorphic in both populations, with two to 18… 
3 Citations

Tables from this paper

Chaotic genetic structure and past demographic expansion of the invasive gastropod Tritia neritea in its native range, the Mediterranean Sea
Past demographic history and contemporaneous genetic structure in the native area of the gastropod Tritia neritea are investigated, using Bayesian skyline plots, multivariate analyses and Bayesian clustering, to highlight a mixture of ancient and recent processes.
Development and cross-amplification of nine polymorphic microsatellite markers in the deep-sea hydrothermal vent polychaete Branchipolynoe seepensis
Cross-amplification tests in other polynoid species revealed that most loci could also be useful to study closely related species from both Atlantic and Pacific sites.
A method for genotype validation and primer assessment in heterozygote-deficient species, as demonstrated in the prosobranch mollusc Hydrobia ulvae
The simple method described to identify unreliable markers will prove a useful technique for many population studies, and also emphasises the dangers in using a single primer set and assuming marker reliability in populations shown to naturally deviate from HWE.


Invasion of the eastern Bay of Biscay by the nassariid gastropod Cyclope neritea: origin and effects on resident fauna
Molecular genetic analyses based on mitochondrial markers suggest that the present C. neritea population in Arcachon Bay has been introduced, probably unintentionally with oyster transfers, from several source populations, genetically similar to the populations analysed in this study, i.e. those in the western Mediterranean and in south Portugal.
Does global change increase the success of biological invaders?
  • Dukes, Mooney
  • Environmental Science
    Trends in ecology & evolution
  • 1999
Biological invasions as a component of global change in stressed marine ecosystems.
Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles.
A method is described for the rapid isolation of microsatellite sequences using a biotin-labeled oligonucleotide attached to streptavidin-coated magnetic particles that is applicable to any genome and in principle is adaptable to the rapid isolated of both repetitive as well as genic sequences.
Primer3 on the WWW for general users and for biologist programmers.
This chapter assumes acquaintance with the principles and practice of PCR, as outlined in, for example, refs.
Testing heterozygote excess and deficiency.
Different exact tests, appropriate for small sample size and large number of alleles, are proposed in this perspective, and their properties are evaluated by power comparisons.
Microsatellite-enriched libraries : applied methodology for the development of SSR markers in tropical crops
L'etude presentee donne une technique pour developper facilement des banques de marqueurs microsatellites specifiques des plantes tropicales en attendant que la communaute internationale entreprenne des projets moleculaires appliques a l'amelioration genetique of ces cultures traditionnellement oubliees.